The workflow includes the processing of both RNA and DNA libraries from a single sample over the course of two days (Figure 1). The Illumina TruSight® Tumor 170 Panel covers 170 gene targets associated with solid tumors.
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This laboratory supports multiple disciplines and also serves as a development hub for molecular testing, allowing the lab to serve Miami Cancer institute’s patients with cutting edge technology and expertise in routine as well as specialized esoteric molecular testing.įigure 1: Illumina TruSight® Tumor 170 Manual Workflow 1
The laboratory serves the patients at Miami Cancer Institute as well as patients throughout Baptist Health South Florida. The Molecular Diagnostics Laboratory is a clinical laboratory accredited by CLIA and CAP and provides cancer genomic and routine molecular testing, by means of complex molecular analyses in oncology and bone marrow transplantation. Miami Cancer Institute is Florida's only member of the Memorial Sloan Kettering Cancer Alliance. Miami Cancer Institute of Baptist Health South Florida is a new state-of-the-art facility which brings outpatient cancer services together under one roof to offer world-class clinical services and cutting-edge therapies. Here we describe the automation of the MCI Advantage Cancer Panel at Miami Cancer Institute, a 170 gene panel assay based upon the Illumina TruSight® Tumor 170 Panel, on the Beckman Coulter Biomek i5 Span-8 NGS Workstation.
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CytoFLEX Violet-Blue-Red Series Upgrades.Navios EX – Powerful, Dependable Flow Cytometer.The efficiency, sensitivity, and specificity of the DxH 800 were 77.0%, 87.1%, and 73.0%, respectively, compared with the Sapphire at 75.8%, 93.5%, and 68.8%, respectively, and LH 780 at 66.1%, 93.5%, and 55.3%, respectively. We also compared clinical efficiency by determining whether flagged specimens showed abnormalities on a peripheral blood smear as defined by International Council for Standardization in Haematology criteria. In the instrument vs 400-cell manual differential comparison, all 3 instruments showed similar and acceptable accuracy to the reference method except for nucleated RBC (NRBC) enumeration, in which the DxH 800 and Sapphire outperformed the LH 780. The DxH 800 provided a CBC and differential that correlated well with those of the Sapphire and LH 780, with most parameters showing correlation coefficients (r) of more than 0.97. We evaluated the new Beckman Coulter DxH 800 hematology analyzer (Beckman Coulter, Miami, FL) vs the Abbott Diagnostics Cell-Dyn Sapphire (Abbott Diagnostics, Santa Clara, CA) and Beckman Coulter LH 780 hematology analyzers using 430 adult specimens.